Cotranslational Protein Expression System for High-Throughput Screening
Lead Inventor: James Inglese (NCATS)
Inventors: Chih-Chien (Ken) Cheng (NCATS), Samuel Hasson (NCATS)
Ref. No.: E-300-2012/0
Abstract: Reporter gene-based assays are used extensively in high-throughput screening to identify chemical modulators of cellular pathways for drug discovery and development. However, such screening frequently results in a large number of false “hits” due to interactions of screened compounds with reporter proteins, producing confounding results. Thus, validation of results using these assays often involves significant time and expense.
The inventors have developed an assay system that significantly improves detection of target-relevant active compounds by discriminating between signals arising from the target activity and those caused by reporter bias.
A Coincidence Reporter-Gene System for High-Throughput Screening • Nature Methods • October 2012 • NCATS Chemical Genomics Center