B-Roll

Description

Preview

Primary screening system 

Arms 1 and 3 on the Primary screening system are working simultaneously on parallel processes. Microtiter plates are loaded onto the necessary devices, an operation is performed per the associated protocol and they are returned to the original storage location.

Runtime: 1:36
Date: 2021
Please contact NCATS to obtain the raw video footage files.
Credit: NCATS

Acoustic compound dispense and assay detection protocols running simultaneously 

Multiple plates are acoustically dispensed with nanoliters of chemical compounds. Shows robotic arm 1 unloading assay and chemical compound source plates from incubator and storage hotel respectively, removing the metal lids and placing onto the acoustic dispenser. This also includes the robotic arm retrieving the plates once finished, replacing lid and loading back into storage locations. In the background arm 3 is also working to process plates for an assay detection protocol.

Runtime: 9:26
Date: 2021
Please contact NCATS to obtain the raw video footage files.

Credit: NCATS

Assay detection protocol using arm 3 of Primary screening system 

1536-well assay plates containing cells and chemical compounds are being loaded onto a solenoid valve low volume liquid dispenser, after the metal lid is removed, which is adding microliters of a detection reagent into every well. The lid is then put back on and the assay plate is loaded into a storage hotel for incubation. The final step in the protocol is removing the lid and loading the plate into the multimode reader to collect the resultant data.

Runtime: 4:06
Date: 2021
Please contact NCATS to obtain the raw video footage files.

Credit: NCATS

Automated tissue culture robot and Arm 1 of Primary screening system 

In the background the automated tissue culture platform is running a process where you see the arm within going through specific steps. Simultaneously arm 1 of the Primary screening system is unloading the assay plate as well as the chemical compound acoustic source plate into their respective locations.

Runtime: 2:49
Date: 2021
Please contact NCATS to obtain the raw video footage files.

Credit: NCATS

Acoustic dispense and plate unload/load 

Nanoliters of a library of chemical compounds are dispensed using acoustic energy into a 1536-well assay plate. Once it finishes the dispense the lifter presents the plate and the robotic arm unloads the current plate, replaces the metal lid and returns it to the incubator. The next assay plate in the queue is loaded onto the acoustic dispenser after the lid is removed by the robotic arm.

Runtime: 2:11
Date: 2021
Please contact NCATS to obtain the raw video footage files.

Credit: NCATS

End of cell splitting protocol 

Cells added into a new flask, returned to incubator and pipette disposed of by robotic arm.

Runtime: 1:13
Date: 2021
Please contact NCATS to obtain the raw video footage files.
Credit: NCATS

Automated tissue culture robot 

Reagents are being dispensed and emptied from a cell flask as part of an automated cell splitting process run on the system which also includes removing and replacing the flask cap as needed and perturbing to wash flask inner surface thoroughly.

Runtime: 2:39
Date: 2021
Please contact NCATS to obtain the raw video footage files.

Credit: NCATS

Plate incubator carousel rotating 

Assay plate incubator carousel is rotating and shows the 1536-well white microtiter plates with metal lids to prevent evaporation stored within.

Runtime: 0:19
Date: 2021
Please contact NCATS to obtain the raw video footage files.
Credit: NCATS

Acoustic chemical compound dispense 



Runtime: 1:15
Date: 2021
Please contact NCATS to obtain the raw video footage files.
Video courtesy of NIH's National Human Genome Research Institute.

Tox21 robot b-roll

B-roll of the high-speed robot screening system for the Toxicology in the 21st Century initiative.

Runtime: 9:20
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

NCATS Chemical Genomics Center (right-to-left pan)

Right-to-left pan of the front of the NCATS Early Translation Branch’s chemical genomics production line showing chemical prep area, incubator and library carousel of compounds.

Runtime: 0:20
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Three Tox21 robots (extreme wide shot)

A tilt-down wide shot showing three yellow robot arms moving plates in the testing production line.

Runtime: 0:31
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Front of production line (extreme wide angle)

A wide angle establishing a shot of the front of the production line.

Runtime: 0:13
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Robots two and three working (medium shot)

A medium shot of robots two and three moving plates along the production line.

Runtime: 0:18
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Two robots moving (medium shot)

Medium tilt-down shot of robots one and two moving plates along the production line.

Runtime: 0:26
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Multiple plates moving (wide shot)

Wide shot, from an angle, of two robots moving plates along the production line.

Runtime: 0:34
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Two robots exchanging plates (wide shot)

Wide shot, from the side, showing two robots moving plate across transfer platform.

Runtime: 0:19
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Transfer plate (extreme close-up)

Extreme close-up of a transfer plate.

Runtime: 0:14
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Tilt-down feeding the detector (close-up)

Close-up tilt-down shot of a robot arm placing a reaction plate on a detector that measures the reactions.

Runtime: 0:23
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Microfluidics pipetting machine (extreme close-up to wide shot)

Extreme close-up pulls back show a microfluid pipetting machine that puts chemicals in plates.

Runtime: 0:18
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Robot feeding stacking rack (wide shot with pan)

Wide shot of a robot arm moving a plate to a stacking rack.

Runtime: 0:09
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Control panel (wide shot)

Wide shot of the control panel for the production line with a robot arm moving in the background.

Runtime: 0:12
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Chemistry station (wide shot)

Wide shot of the chemistry station where chemicals are mixed and added to the pipeline with the robot moving in the background.

Runtime: 0:16
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Molecular libraries (wide shot)

Static wide shot showing the molecular libraries end of the production line with a library carousel rotating.

Runtime: 0:11
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Two robots feed microfluidics system (medium shot)

Medium shot of two robots moving plates across the transfer station in front of a fluid-handling system.

Runtime: 0:12
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Molecular library carousel (medium shot)

Medium shot of the molecular library carousel rotating to make volumes available to production line.

Runtime: 0:08
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Fluid handling (wide shot)

Wide shot of the fluid supply and handling end of the production line.

Runtime: 0:15
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Technician cleaning sensor (wide shot)

Medium, over-the-shoulder shot of a technician cleaning the sensor on a bench-top lab machine.

Runtime: 0:07
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Technician cleaning plates (wide shot)

Wide shot of a technician removing reaction plates from tray and cleaning them on a lab bench.

Runtime: 0:20
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Technician laying out plates (wide shot to medium shot)

Wide shot of a technician laying out plates on lab bench, with a zoom in to a medium shot as he cleans the plates.

Runtime: 0:33
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.

Technician cleaning many plates (extreme close-up)

Extreme close-up of a technician cleaning reaction plates on a lab bench.

Runtime: 0:10
Date: 2010
Video courtesy of NIH's National Human Genome Research Institute.