TNRF Scientific Capabilities

Historically, a lack of methods to properly interpret the results of genome-wide screens, a lack of collaborative expertise to perform RNAi experiments, and the absence of comprehensive RNAi data in public databases for researchers to reference all have limited RNAi’s usefulness. To address these problems, NCATS operates a state-of-the-art RNAi and CRISPR screening facility open to NIH investigators.

NCATS staff assist NIH investigators with all stages of project planning and execution, from assay development through genome-wide siRNA screens, informatics and pathway analysis, and rigorous confirmation of results. TNRF experts routinely perform common seed analysis, multiple siRNA testing and separation of false positives from true positives and are extending this platform to develop similar methodologies for CRISPR/Cas9-based screening.

Learn more about TNRF’s available resources and expertise.