HHS Logo U.S. Department of Health & Human Services Divider arrow NIH logo National Institutes of Health Alt desc
Skip Over Navigation Links
Staff banner

Staff Profile: Nathan Coussens

Nathan P. Coussens, Ph.D.

Senior Research Scientist, Biology (Contractor)

Division of Pre-Clinical Innovation

National Center for Advancing Translational Sciences

National Institutes of Health

E-mail Nathan P. Coussens

Biography

Nathan P. Coussens joined NCATS in 2013 as a research scientist. He also serves as the editor-in-chief of the Assay Guidance Manual. Coussens maintains a diverse target portfolio and is part of a collaborative, interdisciplinary team of scientists and engineers focused on developing small molecule probes from active compounds discovered through high-throughput screening.

Coussens received a Ph.D. in biochemistry in 2007 from the University of Iowa, where he combined structural and biophysical approaches to study host-pathogen interactions. As a fellow of the Immunology Postdoctoral Training Program, Coussens joined a collaboration working to develop novel therapeutics targeting a host evasion mechanism exploited by families of pathogenic bacteria. He contributed to the structure-assisted design of small molecule inhibitors for sialic acid-binding protein from nontypeable Haemophilus influenzae.

As a postdoctoral fellow in the laboratory of Lawrence E. Samelson, M.D., at the National Cancer Institute, Coussens used a multi-angle approach to study signaling in T lymphocytes. His work used biophysical techniques to characterize interactions between critical proteins that transduce signals initiated by the T-cell antigen receptor. Coussens further generated stable cell lines and carried out biological studies and high-resolution imaging to demonstrate the relevance of protein interactions to signaling in T cells.

Research Topics

Coussens’ research interests include biochemical and cell-based assay development and optimization, novel approaches to assay design, and rare and neglected diseases. Specific interests include:

  • Antibiotic resistant bacteria
  • Inflammatory diseases
  • Oncology targets
  • Immunotherapy approaches
  • Epigenetic regulators
  • Metabolic disorders

Coussens aims to use biologically and pharmacologically relevant assays to facilitate the identification of active compounds from high-throughput screens to serve as starting points for molecular probe development. Moreover, he is committed to driving the advancement of such compounds into pre-clinical research and studies to enable submission of Investigational New Drug applications for novel therapeutics development.

Selected Publications

  1. The linker for activation of T cells (LAT) signaling hub: from signaling complexes to microclusters.
  2. Multipoint binding of the SLP-76 SH2 domain to ADAP is critical for oligomerization of SLP-76 signaling complexes in stimulated T cells.
  3. Strategies for assessing proton linkage to bimolecular interactions by global analysis of isothermal titration calorimetry data.
  4. Ablation of Smurf2 reveals an inhibition in TGF-β signalling through multiple mono-ubiquitination of Smad3.
  5. Characterization of the N-acetyl-5-neuraminic acid-binding site of the extracytoplasmic solute receptor (SiaP) of nontypeable Haemophilus influenzae strain 2019.