Nuclear receptors (NRs) play critical roles in human development, metabolic homeostasis and reproduction. Inappropriate activation of nuclear receptors by environmental chemicals can lead to a broad spectrum of negative health effects. To evaluate environmental chemicals’ effects on endocrine disruption, Tox21 researchers have selected appropriate NR assays for quantitative high-throughput screening (qHTS).
As a proof-of-concept study, researchers profiled in qHTS format approximately 3,000 environmental chemicals against a panel of 10 human NRs during the Tox21 pilot phase. The team evaluated these data from a chemical genomics point of view, in addition to assessing data reproducibility as a measure of data quality and applicability for down-stream analyses.