ADST program experts have a documented track record of developing entry-point high-throughput screening assays as well as secondary assays using state-of-the-art tools, technologies, platforms and chemical libraries.
Assay Design Concepts and Proof-of-Concept Testing
This approach employs multimodal outputs adaptable to a variety of biological targets and pathways and can be used to construct assays that phenocopy genetically inherited diseases.
Concentration Response-Based Chemogenomic Profiling
This methodology for high-confidence primary data sets involves micro-volume (3–5 uL) concentration-response determinations of compound series or small libraries for cell-based or biochemical assays.
Collaborative Assembly of Preliminary Validation Studies
This work with high-throughput screening assays supports grant applications or other types of program needs of extramural collaborators.
- Approved drugs for repurposing
- Pharmacologically active compounds for chemical genomic-based target assessment
- Novel, high-stereogenic carbon content libraries
- Collections of culturable pre-fractionated natural product extracts
- Marine natural product extracts and natural product–inspired synthetic collections (e.g., chemical methodology library development) from academic collaborators
- Molecular target–focused and pathway-annotated chemical libraries
Detectors and reagent dispensers for a comprehensive variety of assay techniques and formats, including:
- NCATS-developed high-throughput screening–compatible coincidence reporter systems
- Laser scanning cytometry and automated microscopy-enabled high-content assays
- Target-based, purified-component assays
Informatics Analysis Tools
- Commercial, open-source and custom bioinformatics and cheminformatics software and analysis algorithms
- More about informatics at NCATS
- A broad network of internal and external collaborators and expertise for early-stage translational research