Skip to main content
HHS Logo U.S. Department of Health & Human Services Divider arrow NIH logo National Institutes of Health Alt desc
Toxicology in the 21st Century

Tox21 Expertise

Tox21 experts have expertise in the following areas:

Large-Scale Screening

  • Assay development
  • Assay design
  • Assay validation (assay screened in triplicate against pharmacologically active compounds and 88 additional compounds selected by the Tox21 Chemical Selection Group to evaluate reproducibility and positive control consistency)
  • Assay optimization
  • High-content screening
  • Quantitative high-throughput screening (qHTS)
  • Assay miniaturization (adapted for the qHTS platform)
  • Gene expression screening platform (RASL-seq)

Data Analysis

  • Normalization and correction
  • Concentration-response curve-fitting to generate potency and efficacy
  • Classification of curves based on significance of fit (by p-values)
  • Completeness of fit
  • Efficacy

Data Dissemination

Data available in these public databases encourage independent evaluations of Tox21 findings: